Vladimir Sergienko was born in 1942 in Moscow. After graduating from the Moscow Medical dental Institute in 1966, he worked as a doctor in Stroginskoy district hospital in Vladimir region. In 1968, V. Sergienko came to the innovative for the domestic medical science industry – radionuclide diagnostics. From 1968 to 1973 he worked in the radioisotope laboratory of the all-Union research Institute of gastroenterology (Director – academician V. H. Vasilenko). In 1972 he defended his thesis on “Quantitative assessment of scanogram in chronic liver diseases". From 1973 to 1979 he was a senior researcher in the Department of Clinical physiology of the Sklifosovsky research Institute of emergency medicine, the head of the newly established in 1973 laboratory for the radioisotope. In 1979 he was appointed head of the Department of radioisotope diagnostics of the Central clinical hospital of the Fourth main Department under the Ministry of health of the USSR. In 1984, Vladimir Sergienko was invited by academician E. I. Chazov to head the Department of radionuclide diagnostics of the scientific research Institute of Clinical cardiology named A. L. Myasnikov.

In the period from 1980 to 1983, Vladimir Borisovich was the first in the USSR to introduce into clinical practice a new diagnostic method-single-photon emission computed tomography. Advanced work Vladimir Sergienko, devoted to the possibilities of using single-photon emission computed tomography in cardiology, pulmonology, neurology, gastroenterology and endocrinology, published in 1980-1985 in the leading scientific journals of the country, became fundamental for the domestic radionuclide diagnosis and radiology in General, and formed the basis of his doctoral thesis on "single-Photon emission computed tomography. Clinical experience" (1985).

In 1988, Vladimir Sergienko was awarded the title of Professor. In 1991, the team of authors, Vladimir Sergienko awarded the Prize of the Council of Ministers of the USSR for the development of a new radiopharmaceutical – 201Tl-chloride of thallium, up to now widely used as a marker of viability and impaired cellular perfusion of the myocardium. In 2000-h years Vladimir Borisovich was elected the Chairman of the Moscow society of radioisotope diagnosis, he worked as a freelance Chief radiologist of Ministry of health of the Russian Federation, adviser on medicine, Head of the Federal Agency of the Russian Federation for atomic energy. He was appointed Chairman of the NTS Rosatom, worked as an expert of the Higher Attestation Commission. In 2010, on the initiative of the management of the research center ”Kurchatov Institute” was appointed coordinator of the working group on the development of the concept of nuclear medicine in the country. Since 2015, he has been an expert of the Russian Academy of Sciences in nuclear medicine.

Vladimir Sergienko has been conducting intensive scientific and pedagogical work for many decades, is the author of more than 400 scientific publications, including 8 patents and 4 monographs, a number of methodological recommendations for radionuclide diagnostics methods. Under his leadership, a number of new radiopharmaceuticals have been developed and experimental studies are continuing on the early diagnosis of pathological processes in cardiovascular diseases. Vladimir Borisovich is a member of the European society of nuclear medicine (EANM), a member of the Board of the society of Nuclear medicine, is a member of the editorial boards of the journals “Journal of Radiology and nuclear madicine”, ”Medical Physics” and “cardiology Bulletin”. Vladimir Borisovich is the head and consultant of 6 doctoral and 53 master's theses, many of his students are now key experts in the field of domestic nuclear medicine.